[生物資訊期末報告]The analysis for nucleotide/protein sequence(IRF-1)

        10         20         30         40         50         60
ATGCCCATCA CTCGGATGCG CATGAGACCC TGGCTAGAGA TGCAGATTAA TTCCAACCAA 

        70         80         90        100        110        120
ATCCCGGGGC TCATCTGGAT TAATAAAGAG GAGATGATCT TCCAGATCCC ATGGAAGCAT 

       130        140        150        160        170        180
GCTGCCAAGC ATGGCTGGGA CATCAACAAG GATGCCTGTT TGTTCCGGAG CTGGGCCATT 

       190        200        210        220        230        240
CACACAGGCC GATACAAAGC AGGGGAAAAG GAGCCAGATC CCAAGACGTG GAAGGCCAAC 

       250        260        270        280        290        300
TTTCGCTGTG CCATGAACTC CCTGCCAGAT ATCGAGGAGG TGAAAGACCA GAGCAGGAAC 

       310        320        330        340        350        360
AAGGGCAGCT CAGCTGTGCG AGTGTACCGG ATGCTTCCAC CTCTCACCAA GAACCAGAGA 

       370        380        390        400        410        420
AAAGAAAGAA AGTCGAAGTC CAGCCGAGAT GCTAAGAGCA AGGCCAAGAG GAAGTCATGT 

       430        440        450        460        470        480
GGGGATTCCA GCCCTGATAC CTTCTCTGAT GGACTCAGCA GCTCCACTCT GCCTGATGAC 

       490        500        510        520        530        540
CACAGCAGCT ACACAGTTCC AGGCTACATG CAGGACTTGG AGGTGGAGCA GGCCCTGACT 

       550        560        570        580        590        600
CCAGCACTGT CGCCATGTGC TGTCAGCAGC ACTCTCCCCG ACTGGCACAT CCCAGTGGAA 

       610        620        630        640        650        660
GTTGTGCCGG ACAGCACCAG TGATCTGTAC AACTTCCAGG TGTCACCCAT GCCCTCCACC 

       670        680        690        700        710        720
TCTGAAGCTA CAACAGATGA GGATGAGGAA GGGAAATTAC CTGAGGACAT CATGAAGCTC 

       730        740        750        760        770        780
TTGGAGCAGT CGGAGTGGCA GCCAACAAAC GTGGATGGGA AGGGGTACCT ACTCAATGAA 

       790        800        810        820        830        840
CCTGGAGTCC AGCCCACCTC TGTCTATGGA GACTTTAGCT GTAAGGAGGA GCCAGAAATT 

       850        860        870        880        890        900
GACAGCCCAG GGGGGGATAT TGGGCTGAGT CTACAGCGTG TCTTCACAGA TCTGAAGAAC 

       910        920        930        940        950        960
ATGGATGCCA CCTGGCTGGA CAGCCTGCTG ACCCCAGTCC GGTTGCCCTC CATCCAGGCC 

       970
ATTCCCTGTG CACCGTAG

Number of amino acids: 978

Molecular weight: 79750.5

Theoretical pI: 5.04
Amino acid composition: 
Ala (A) 261	 26.7%
Arg (R)   0	  0.0%
Asn (N)   0	  0.0%
Asp (D)   0	  0.0%
Cys (C) 265	 27.1%
Gln (Q)   0	  0.0%
Glu (E)   0	  0.0%
Gly (G) 269	 27.5%
His (H)   0	  0.0%
Ile (I)   0	  0.0%
Leu (L)   0	  0.0%
Lys (K)   0	  0.0%
Met (M)   0	  0.0%
Phe (F)   0	  0.0%
Pro (P)   0	  0.0%
Ser (S)   0	  0.0%
Thr (T) 183	 18.7%
Trp (W)   0	  0.0%
Tyr (Y)   0	  0.0%
Val (V)   0	  0.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 0
Total number of positively charged residues (Arg + Lys): 0

Atomic composition:

Carbon      C	      2848
Hydrogen    H	      4720
Nitrogen    N	       978
Oxygen      O	      1162
Sulfur      S	       265

Formula: C2848H4720N978O1162S265
Total number of atoms: 9973

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16500
Abs 0.1% (=1 g/l)   0.207, assuming all pairs of Cys residues form cystines

Ext. coefficient        0
Abs 0.1% (=1 g/l)   0.000, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 48.90
This classifies the protein as unstable.

Aliphatic index: 26.69

Grand average of hydropathicity (GRAVY): 0.917